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Literature summary extracted from
- Proteolytic specificity and cobalt exchange of hemorrhagic toxin e, a zinc protease isolated from the venom of the western diamondback rattlesnake (Crotalus atrox) (1983), Biochemistry, 22, 3770-3778.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.44 | EDTA | insulin A-chain | Crotalus atrox |  |
| 3.4.24.44 | additional information | no inhibition by aprotinin (insulin A-chain as substrate) | Crotalus atrox |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.44 | Co2+ | can be directly exchanged for zinc by dialysis without loss of specificity or activity | Crotalus atrox | |
| 3.4.24.44 | Co2+ | no conformational change accompanies the metal exchange | Crotalus atrox | |
| 3.4.24.44 | Zn2+ | zinc metalloproteinase | Crotalus atrox | |
| 3.4.24.44 | Zn2+ | zinc removal causes large structural perturbations replacing it with Co2+ is accomplished without structural changes | Crotalus atrox | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.44 | Crotalus atrox | - |
Western diamondback rattlesnake | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.44 | venom | - |
Crotalus atrox | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.44 | Dimethylcasein + H2O | - |
Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | Oxidized insulin A-chain + H2O | rapid cleavage at Tyr14-Gln15, more slowly at Ala8-Ser9, kinetics | Crotalus atrox | Hydrolyzed oxidized insulin A-chain | peptides (Gln15-Asn21) + (Gly1-Tyr14) + (Ser9-Tyr14) + (Gly1-Ala8) | ? | |
| 3.4.24.44 | Oxidized insulin B-chain + H2O | cleavage sites: Ser9-His10, Ala14-Leu15 (very rapidly) | Crotalus atrox | Hydrolyzed oxidized insulin B-chain | - |
? | |
| 3.4.24.44 | Oxidized insulin B-chain + H2O | kinetics | Crotalus atrox | Hydrolyzed oxidized insulin B-chain | - |
? | |
| 3.4.24.44 | Oxidized insulin B-chain + H2O | cleavage sites: Asn3-Gln4 (very slowly) | Crotalus atrox | Hydrolyzed oxidized insulin B-chain | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.44 | 37 | - |
assay at | Crotalus atrox |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.44 | 0.00767 | - |
oxidized insulin A-chain | Ala8-Ser9 bond | Crotalus atrox | |
| 3.4.24.44 | 0.0433 | - |
oxidized insulin A-chain | Tyr14-Gln15 bond, cobaltous toxin | Crotalus atrox | |
| 3.4.24.44 | 0.06 | - |
oxidized insulin A-chain | Tyr14-Gln15 bond | Crotalus atrox | |
| 3.4.24.44 | 0.167 | - |
dimethylcasein | cobaltous toxin | Crotalus atrox | |
| 3.4.24.44 | 0.217 | - |
dimethylcasein | - |
Crotalus atrox |
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